Insulin Glargine in Type 1 Diabetes Mellitus: A Review of Clinical Trials and Real-world Evidence Across Two Decades

Background Over the past two decades, insulin glargine 100 U/mL (Gla-100) has emerged as the “standard of care” basal insulin for the management of type 1 diabetes mellitus (T1DM). Both formulations, insulin glargine 100 U/mL (Gla-100) and glargine 300 U/mL (Gla-300) have been extensively studied against various comparator basal insulins across various clinical and real-world studies. In this comprehensive article, we reviewed the evidence on both insulin glargine formulations in T1DM across clinical trials and real-world studies. Methods Evidence in T1DM for Gla-100 and Gla-300 since their approvals in 2000 and 2015, respectively, were reviewed. Results Gla-100 when compared to the second-generation basal insulins, Gla-300 and IDeg-100, demonstrated a comparable risk of overall hypoglycemia, but the risk of nocturnal hypoglycemia was higher with Gla-100. Additional benefits of Gla-300 over Gla-100 include a prolonged (>24-hours) duration of action, a more stable glucose-lowering profile, improved treatment satisfaction, and greater flexibility in the dose administration timing. Conclusion Both glargine formulations are largely comparable to other basal insulins in terms of glucose-lowering properties in T1DM. Further, risk of hypoglycemia is lower with Gla-100 than Neutral Protamine Hagedorn but comparable to insulin detemir.


INTRODUCTION
Insulin replacement therapy is the cornerstone for the maintenance of optimal glucose levels and circumventing long-term complications in people with type 1 diabetes mellitus (T1DM) [1,2].Various insulin regimens, including basal bolus (BB), split mixed, premixed, and prandial therapy, are available, which can be individualized based on the profile of the individual with T1DM.However, the use of BB insulin therapies is recommended as the standard regimen for management or type 1 diabetes [2,3].
*Address correspondence to this author at the Department of Endocrinology, Diabetes Care & Hormone Clinic, Ahmedabad, Gujarat, India; E-mails: banshisaboo98@gmail.com; banshisaboo@hotmail.com 1875-6417/ 24 Among the available basal insulins (BIs), insulin glargine has emerged as one of the standard choices among clinicians for the management of T1DM [4].Presently, it is available in two formulations; insulin glargine 100 U/mL (Gla-100) and the second-generation BI analog insulin glargine 300 U/mL (Gla-300).Over the last two decades, various clinical and real-world studies have evaluated the glargine formulations, thereby creating significant evidence base on their efficacy/effectiveness and safety profile in people with T1DM [5,6].This article aims to provide a comprehensive review of the evidence from clinical and real-world studies in T1DM on the use of glargine, i.e.Gla-100 or Gla-300, since its approval in the year 2000 and 2015, respectively.

METHODOLOGY
A PubMed and Google Scholar search using keywords "insulin glargine", "diabetes mellitus, type 1"[MeSH Terms], "type 1 diabetes mellitus", T1DM, "insulin dependent diabetes mellitus" and "IDDM" was performed to screen relevant articles.Further, a grey literature search was performed using the same search terms.In addition, meta-analyses were screened to identify articles of interest.Articles published resulting from these searches in the last 20 years and relevant references cited in those articles were examined.Only the relevant articles published in English were included.Articles on biosimilar Gla-100 were excluded.From the results, clinical studies that evaluated safety and/or efficacy of Gla-100 and/or Gla-300 versus (vs.) other insulin comparators in people with T1DM were selected for this manuscript.
Gla-300, the second-generation BI analog, has a relatively more compact depot in the subcutaneous tissues, resulting in slower, yet more constant dissolution rate, leads to a more stable and prolonged activity profile compared to Gla-100 [5].The evidence comparing Gla-100 and Gla-300 against various BIs in various clinical and real-world studies in T1DM have been elaborated in subsequent sections.

Glucose-lowering Efficacy and Glycemic Variability
Gla-300 has been evaluated against Gla-100 in multiple clinical trials in T1DM (Table 2) [19][20][21][22][23][24][25][26][27][28][29][30][31].In both EDITION-4 and EDITION-JP1 trials, for a comparable glucoselowering efficacy between Gla-300 and Gla-100, the predinner self-monitoring of plasma glucose (SMPG) in EDI-TION-JP1 trial was found to be significantly better with Gla-300 at the 6-month follow-up [26,29].This difference in the pre-injection SMPG levels could be attributed to the prolonged glycemic coverage with Gla-300, as the prandial insulin coverage was similar across both arms in the EDI-TION-JP1 trial [26].In the recent phase 4 studies (TOPI 1 and OPTIMIZE) evaluating the impact of switching from twice-daily BI (predominantly Gla-100) to once-daily Gla-300, consistently better fasting plasma glucose (FPG) reductions have been observed with Gla-300 vs. Gla-100 [21,22].These observations are indicative of the role of Gla-300 in the T1DM population, who require twice-daily BI.Pertaining to glycemic variability (GV), a trend of lower daily variability was observed with Gla-300 in a small exploratory study (n = 20) from Japan using continuous glucose monitoring (CGM) [27].Additionally, a post-hoc analysis by Bergenstal et al. (n = 59) indicates that a significantly lesser increase in glucose level in the last four hours of each participant's 24-hour injection interval with Gla-300 [24].The remaining GV parameters were comparable between both arms in this study.This trend was also observed in a more recent phase 4 randomized controlled trial by Pettus et al. [23].The authors suggested that the relatively prolonged duration of action may lend to more flexibility in terms of the timing of the BI injection.Additionally, this trial reported a greater time-in-range (TIR) with Gla-300 in people with only glycated hemoglobin (HbA1c) <7.5% at the end-of-study [23].
It is a well-understood phenomenon that the probability of detecting differences in TIR between two different therapies becomes negligible at high HbA1c.Further, TIR becomes significant at lower HbA1c as individuals approach glycemic goals.However, this being only a trend that emerged from a post-hoc analysis, needs further robust evaluation in adequately powered trials.

Hypoglycemia Risk
The multi-national EDITION-4 and Japanese EDITION-JP1 trials revealed a lower risk of nocturnal hypoglycemia, more so during the active titration period and can be attributed to the more stable activity profile of Gla-300 with lesser glycemic variations [26,29].The overall hypoglycemia risk was comparable in the EDITION-4 trial [29].However, the Significant reduction in hypoglycemic events after one year of treatment in individuals with previous hypoglycemic events.
• Gla-300 is a potential basal insulin alternative to treat T1DM, improving metabolic control in individuals with HbA1c levels >7.5 and decreasing hypoglycemic events in individuals with history of hypoglycemia without increasing body weight.
EDITION-JP1 showed a significantly lower risk of overall hypoglycemia with Gla-300, which has been attributed to the difference in lifestyle, diet patterns, and difference in injection timings between both study groups [26].These trends of a lower hypoglycemia risk with Gla-300, especially at night which were also evident in the exploratory CGM studies and the TOP1 study, imply a significant clinical benefit as the protective counter-regulatory mechanisms, which kick in during an episode of severe hypoglycemia, are deficient in people with T1DM, thereby possibly preventing any serious adverse event [21,27,28].

Weight Gain and Insulin Doses
A consistent trend of lesser weight gain was observed in the Gla-300 group vs. Gla-100 in both EDITION trials and the OPTIMIZE study [22,26,29].However, this trend has not been satisfactorily explained in these studies and needs to be explored further, especially considering BI dose with Gla-300 has consistently been higher than Gla-100, with bolus insulin remaining comparable across multiple trials.

Real-world Evidence
Gla-300 has been evaluated in multiple real-world settings, which have largely substantiated the trends observed in clinical trials.Real-world experience from SPARTA study indicated that a change in BI (at baseline: Gla-100, 55% and detemir, 35% of individuals) to Gla-300 was associated with clinically and statistically significant HbA1c improvements, without significant changes in BI dose and weight gain.Low incidence of severe hypoglycemia episodes and related hospitalizations and diabetes ketoacidosis was noteworthy.The rates were still lesser at 6 months post initiation of Gla-300 compared to six months before the treatment.The commonly cited reasons for the switch from previous BIs were lack of efficacy and hypoglycemia concerns, which would be indicative of these two being the most important guiding factors behind the decision about which insulin to initiate.This study revealed a 94% persistence with Gla-300, suggesting good tolerability [31,32].The REALITY study, having people with T1DM switched from Gla-100, NPH, and detemir to Gla-300, also mirrored the effectiveness and safety findings from SPARTA study [32,33].Furthermore, other studies also provide corroborating evidence indicating Gla-300 as a better alternative to Gla-100 to treat T1DM [30,31].In summary, for a comparable HbA1c lowering benefit, Gla-300 is associated with better glycemic coverage, thereby yielding lower pre-dinner SMPG values vs. Gla-100, as well as a significantly greater FPG reduction, especially in a population using 2 daily doses of first-generation basal insulin.Trends suggest Gla-300 has more favorable profile for nocturnal hypoglycemia, more so during the initial eight weeks of active titration, besides being associated with a significantly lower weight gain vs. Gla-100.Initial indications from studies would suggest that both insulins have a good profile on most of the GV parameters, with the advantage of Gla-300's ability to reduce blood glucose levels much better than Gla-100 in the last four hours of the 24-hour injection interval.
A CGM cross-over study from Japan revealed that both Gla-100 and IDeg-100 were observed to be comparable on all GV parameters, with the exception of fasting interstitial glucose, which was significantly higher in individuals receiving Gla-100 vs. those receiving IDeg-100 (P = 0.037).The authors suggested the substantial difference in baseline HbA1c (7.7 ± 0.6% in Gla-100 to IDeg-100 vs. 7.1±0.9% in IDeg-100 to Gla-100; P = 0.077) indicated a worse baseline glycemic status in the Gla-100 group during the first treatment period and hence, a possibility of being more prone to within day glucose variations [35].Considering the openlabel study design and small sample size (n=20), the results of this CGM study need to be interpreted with caution but definitely open up the scope for conducting large-scale prospective studies evaluating Gla-100 vs. IDeg-100 in terms of their GV.

Hypoglycemia Risk
IDeg-100 has consistently shown to be associated with a significantly lower risk of nocturnal hypoglycemia compared to Gla-100, with additional benefits of significantly lower risk of overall confirmed and severe hypoglycemia risk evident in hypoglycemia prone population as seen in SWITCH 1 study [34,[36][37][38].The BEGIN Basal Bolus Type 1 study revealed that IDeg-100 was observed to be associated with a significantly lower risk of nocturnal hypoglycemia (estimated rate ratio of IDeg-100: Gla-100 at 0.75 [95% CI: 0.59, 0.96; P = 0.021]), which was conserved even at the end of a 2-year follow-up period [34,38].However, the overall confirmed, diurnal confirmed and severe hypoglycemic episodes between Gla-100 and IDeg-100 arms were comparable (P > 0.05), irrespective of the titration and maintenance phase [34].These trends were replicated in the BEGIN: Flex T1 trial as well [36].However, the SWITCH 1 study, which included a population with prior history of hypoglycemia episodes, revealed a significantly lower risk of overall symptomatic hypoglycemia with an estimated rate ratio of 0.89 (95% CI: 0.85, 0.94; P < 0.001) for IDeg-100 compared to Gla-100 [37].The trend of significantly lower nocturnal as well as severe hypoglycemia risk with IDeg-100 observed in the BEGIN studies was maintained in the SWITCH 1 study.Iga et al., in their CGM study, also revealed that a similar time was spent in hypoglycemia per 24 hours between both Gla-100 and IDeg-100 (P = 0.300); interestingly, this trend was observed even for the time spent in nocturnal hypoglycemia (P = 0.325) [35].However, given the limitations of this study, these initial trends need to be evaluated in large scale prospective studies.

Weight Gain and Insulin Doses
The BEGIN Basal Bolus Type 1 study and the SWITCH 1 study revealed consistent trends towards the lower total, basal, and bolus insulin doses with IDeg-100.The IDeg-100 dose remained stable in the BEGIN Basal Bolus study throughout the treatment period (52 weeks), with the Gla-100 dose increasing from 0.33 to 0.39 U/kg simultaneously [34].Further, the bolus dose increased substantially in the Gla-100 arm, giving an estimated treatment ratio of IDeg-100: Gla-100 of 0.90 (95% CI: 0.83, 0.98; P = 0.016).The bolus insulin requirement remained stable throughout the SWITCH study and the basal dose was 3% lower with IDeg-100 vs. Gla-100, leading to a very clinically minimal yet statistically significant treatment ratio of 0.97 (95% CI: 0.95, 0.99; P = 0.02) and 0.97 (95% CI: 0.95, 0.99; P = 0.01), for BI and total insulin doses, respectively [37].The SWITCH 1 study population included people with T1DM with a previous history of hypoglycemic episodes, who are generally excluded from clinical trials but represent a significant proportion of the population in clinical practice.The risk of weight gain has been observed consistently to be comparable across multiple studies.
In summary, evidence from the clinical trials indicate that for comparable HbA1c lowering efficacy of Gla-100 with IDeg-100, the risk of nocturnal hypoglycemia is significantly lower with IDeg-100 consistently across trials.Moreover, the FPG values, overall confirmed that severe hypoglycemia risk in hypoglycemia prone T1DM individuals is lower with IDeg-100 vs. Gla-100.Further, the dose requirement for IDeg-100 was also noted to be significantly lesser compared to Gla-100.Thus, evidence would suggest that IDeg-100 may be a better basal insulin option compared to Gla-100, with additional benefits in hypoglycemia prone T1DM population.

Gla-300 versus IDeg-100
Pharmacokinetic-pharmacodynamic (PK/PD) evaluation of Gla-300 and IDeg-100 reported a similar glycemic control and PD equivalence during euglycemic clamps after administration of individualized, clinically titrated doses of Gla-300 and IDeg-100 at steady-state.There was a similar even distribution of insulin activity of Gla-300 and IDeg-100 over 24 h postdosing; however, with lower within-day variability for Gla-300 [39].These findings were mirrored in another study where Gla-300 was evaluated with IDeg-100 in a once-daily morning dosing regimen of 0.4 U/kg/day, which could facilitate titration and enable tighter glycemic control with a reduced risk of hypoglycemia [40].However, Heise et al. reported a contrary finding, wherein IDeg-100 exhibited lower day-to-day and within-day variability than Gla-300 and a more stable glucose-lowering effect [25].This was further evidenced in a pooled data analysis by Heise et al., which revealed a significantly lower relative within-day and day-to-day variability for IDeg-100 compared to both Gla-100 and Gla-300 [41].However, interpretation of these results becomes difficult in light of the differences in the study designs and variations within study subjects.Preliminary trends from clinical studies show lesser variability in 24-hour glucose levels with the longer-acting BIs, including Gla-300 and IDeg-100, compared to Gla-100.However, there is limited clinical data on direct head-to-head comparison between Gla-300 and IDeg-100 in terms of GV (Table 2).Miura H et al. compared (n = 46) direct effects of insulin IDeg-100 and Gla-300 on glycemic stability in patients with T1DM [20].This study utilized both the SMBG and CGM.It showed that insulin IDeg-100 and Gla-300 exert largely similar glucosestabilizing effects, except IDeg-100 achieving a significantly lower mean sensor glucose level (P = 0.02), a lower percentage of time above range (TAR) (≥180 mg/dL) (P = 0.03), and a greater percentage of time below range (TBR) (≤70 mg/dL) (P = 0.04) [20].Contrarily, in a larger population studied in OneCARE study (N=220), switch from Gla-100 or detemir to Gla-300 or IDeg-100 revealed a significantly higher nocturnal (00:00-6:00 h) TIR (70-140 mg/dL, P = 0.0210; 70-180 mg/dL, P = 0.018) and lower nocturnal TAR (P = 0.020) with Gla-300 vs. IDeg-100 [42].A significantly greater amount of nocturnal period (00:00-06:00 h) measured in minutes was spent above 180 mg/dL with IDeg-100 vs. Gla-300 (P = 0.006) [42].These findings translated into a smoother nocturnal mean glucose curve with lesser mean nocturnal glycemic excursions (P < 0.05) with Gla-300.The remaining GV parameters, as measured by CGM along with satisfaction parameters, were comparable between both Gla-300 and IDeg-100.Measurement of GV using CGM allows a more thorough assessment of overall glycemic control and supports TIR as a treatment target.Time-in-range profiles can be used to determine the frequency and duration of glycemic excursions and can help healthcare professionals to establish personalized glycemic targets.The recent consensus report highlighted TIR as the most important actionable metric that can be obtained from CGM [43].Overall, there has emerged a necessity for a head-to-head prospective clinical trial in people with T1DM comparing Gla-300 vs. IDeg-100 in glycemic control and GV.Furthermore, head-to-head evaluation of the benefits and risks of different titration rules and/or insulin dosing schedules is also an unmet need.This potential data would indicate both Gla-300 and IDeg-100's performance in daily life and may also help optimize their dosing.The InRange study, a multicentric phase 4 study (NCT04075513), is an ongoing trial that has been designed to address this unmet need and to demonstrate that Gla-300 is non-inferior to IDeg-100 in glycemic control (TIR) and co-efficient of variance (CV) [44].The growing acceptance of CGM would suggest that it may become the new gold standard for the evaluation of glycemic control in the future.In this context, InRange and similar studies utilizing CGM (Table 3) assume significance as they are expected to provide further insights into the utility of CGM as an outcome measure in clinical practice [44][45][46][47].

Glucose-lowering Efficacy
Gla-100 was observed to be comparable to insulin detemir in terms of its glucose-lowering efficacy in multiple studies [18,[48][49][50].The data from the Treat-to-target trial showed comparable glycemic control in both detemir (overall) and Gla-100 arms with the estimated changes in HbA1c from baseline being -0.53% and -0.54%, respectively.Analyzing the detemir arm by the number of doses administered daily showed an HbA1c reduction of -0.49% in the oncedaily detemir and -0.58% in the twice-daily detemir group [49].Though, FPG reduction trends were observed to be variable across multiple studies, Pieber et al. reported that a greater proportion of the individuals receiving Gla-100 achieved their pre-breakfast target plasma glucose (< 130 mg/dL), (67% vs. 58%) [49].Further, both insulins have reported similar SMPG profiles across multiple studies [48].

Hypoglycemia Risk and Glycemic Variability
Both Gla-100 and detemir have reported comparable overall hypoglycemia risk [18,[48][49][50].However, evidence • Gla-100 US Rate of rebound hyperglycemia • Rate of recurrent ketogenesis • Risk of hypoglycemia between those given early administration of glargine vs. those given standard-of-care management.
• Evaluation of CGM and POC glucose monitoring during DKA treatment in children.NCT03952130 • IDeg-100

• Glargine US
Lower beta hydroxybutyrate levels (Hypothesis: beta hydroxybutyrate levels will be lower in the morning (≥0.6 mmol/L) of the first day of the school week in subjects receiving IDeg, since the long halflife of IDeg will compensate for missed insulin doses over the weekend/holidays) • Control of HbA1c levels • Measure of beta hydroxybutyrate levels between the 2 groups (Differences in frequency of elevated beta hydroxybutyrate levels when receiving supervised injections of basal insulin vs. when not receiving supervised injections).
pertaining to nocturnal hypoglycemia risk appears variable.Pieber et al. have reported a 32% higher risk of nocturnal hypoglycemia (P = 0.046) and 72% increased risk of severe hypoglycemia (P = 0.047) with Gla-100 once-daily vs. detemir twice-daily [49].On the contrary, other studies have reported either a comparable risk of nocturnal hypoglycemia or similar amount of time spent in hypoglycemia (<70 mg/dL) [18,48,50].
Pertaining to GV, Heller et al. showed a comparable within-individual variation (Standard Deviation, SD) in prebreakfast and pre-dinner plasma glucose levels [48].Similarly, Tsujino et al. reported comparable GV between Gla-100 and detemir (GV parameters were SD of 24-h glucose levels, mean amplitude of glycemic excursions and mean of daily difference (MODD) [50].However, another study has shown significantly lower GV with Gla-100 vs. detemir (P < 0.05) [18].

Weight Gain and Insulin Doses
Multiple studies show a higher BI dose requirement in the detemir vs. Gla-100 group, with almost similar bolus dose requirement and weight changes [18,[48][49][50].Two previous studies reported similar body weight changes in detemir and Gla-100 groups (Pieber et [48,49].

Real-world Evidence
Various real-world studies have evaluated the effectiveness and tolerance of Gla-100 vs. detemir in routine clinical practice.A study by Renard et al. indicated non-inferiority of Gla-100 to detemir for the primary end point of CV of FPG and comparability of metabolic outcomes, weight variations and hypoglycemic episodes.However, a trend of higher insulin dose requirement and a greater number of injections was observed with detemir [51].Similarly, Abali et al. reported comparable glucose-lowering efficacy but at a significantly higher mean BI dose for detemir (0.52 vs. 0.41 U/kg/d for Gla-100, P < 0.001) [52].The comparable safety of Gla-100 and detemir and their superiority over NPH was attested by a study from Finland that reported a lower crude incidence rate for the first hypoglycemic coma event (per 100 person-years) with insulin detemir or Gla-100 [53].
Overall, the evidence suggests comparable glucoselowering effectiveness in terms of both HbA1c and FPG values between Gla-100 and detemir, but more insulin doses and a number of injections are required with detemir.Further, the more recent data would point towards a comparable hypoglycemia risk, irrespective of time or severity of hypoglycemia as well as similar weight changes with both insulins.Additionally, GV for Gla-100 was observed to be either comparable or lesser than detemir.

Pediatric Population
The USFDA has approved Gla-100 use in adolescents and children aged ≥ 2 years and Gla-300 use in adults and pediatric population aged ≥ 6 years [54,55].The trends observed in the pediatric T1DM studies comparing Gla-100 with NPH and detemir mirror the observations from the adult T1DM studies.A study evaluating the safety and efficacy of Gla-100 vs. NPH in the Chinese pediatric population reported that for comparable HbA1c reductions at week-24, a lower rate of symptomatic hypoglycemia was observed in individuals receiving Gla-100 (24.3 vs. NPH-32.3per patientsyear) [56].Multiple studies comparing Gla-100 vs. NPH insulin in the pediatric T1DM population showed similar trends (Table 4) [52,[56][57][58][59][60].Gla-100 vs. detemir, when used as a part of the BB insulin therapy in a retrospective study showed similar glycemic control in both arms, but at an approximately 27% higher BI and 19% higher total daily insulin dose with detemir [52].Hence, while starting BI or switching to detemir from Gla-100 in newly diagnosed children with T1DM, a unit-for-unit conversion from Gla-100 to detemir may not be useful.The EDITION JUNIOR, which was a non-inferiority study of Gla-300 to Gla-100 in children and adolescent (>6 years) T1DM population, showed similar hypoglycemia incidences across both thresholds (< 70 and <54 mg/dL) at comparable HbA1c levels.A non-significant trend was noted for lower severe hypoglycemia rates with Gla-300, though this needs further exploration to derive clinically meaningful results.A similar observation of a numerically lower incidence of hyperglycemia with ketosis (ketone ≥ 1.5 mmol/L) was noted in Gla-300 group.Basis this evidence, Gla-300 has emerged as a suitable option in the pediatric population > 6 years with T1DM [19].Abbreviations: Gla-100, glargine 100 U/mL; Gla-300, glargine 300 U/mL; HbA1c, glycated hemoglobin; NPH, neutral protamine Hagedorn; NR, not reported; T1DM, type 1 diabetes mellitus.

Pregnancy
A meta-analysis of eight observational clinical studies of Gla-100 vs. NPH in gestational or pre-gestational diabetes (Gla-100, n = 331; NPH, n = 371) suggested no significant differences in safety-related maternal or neonatal outcomes [61].Post-marketing surveillance data indicated congenital anomalies and spontaneous abortions with both Gla-100 and Gla-300 were extremely rare and mirrored the general population [62].Another meta-analysis reported Gla-100 as a safe treatment option for diabetes during pregnancy [63].Furthermore, a transplacental transfer study found that Gla-100 does not cross the human placenta at therapeutic concentrations, indicating that Gla-100 can be used safely during pregnancy [64].

Fasting during Ramadan
Prolonged use of insulin in people with T1DM during Ramadan carries a risk of severe hypoglycemia, hyperglycemia, and ketoacidosis due to the long hours of fasting.The IDF-Diabetes and Ramadan International Alliance (IDF-DAR) consensus recommends the use of long-acting BI analogs during fasting, considering the low risk of hypoglycemia [65].Insulin Gla-100 used as the BI resulted in excellent control in 15 non-exercising individuals who fasted for 18 hours, with mean plasma glucose of 5.1-6.9mmol/L during fasting [66].In insulin receiving individuals with T1DM who are fasting during Ramadan, Al-Arouji et al. recommended one injection of Gla-100 or two injections of detemir along with a pre-meal rapid-acting insulin analog as an alternative in insulin-treated individuals with T1DM who fast during Ramadan.[67].Further, the IDF-DAR recommends reducing BI dose by 15% for a once-daily dose regimen; for a twice-daily regimen, the usual dose may be taken at iftar with a 50% reduction at suhoor.Dose titration of BI is recommended to be performed every three days on the basis of the pre-iftar blood glucose values with the aim of maintaining blood glucose levels between 90 and 126 mg/dL [65].

Renal Impairment
Data collected by Hasslacher et al. of 509 T1DM individuals revealed an unadjusted median estimated glomerular filtration rate (eGFR) of 83 mL/min and 94 mL/min in the human insulin (HI) and insulin analog (IA) groups (P = 0.01) [68].The median urine albumin-creatinine ratio (ACR) was 7 mg/g in the HI group compared to 6 in the IA group (P = 0.01), whereas the median hemoglobin (Hb) was 13.9 g/dL and 13.7 g/dL in the HI and IA group respectively (P = 0.02).A multiple linear regression analysis to ascertain the relation between the type of insulins and eGFR, ACR and Hb revealed that besides the overall diabetic population, multiple daily injections treatment with insulin Gla-100 as the basal insulin showed benefits in people with T1DM having impaired renal function.The use of Gla-100 based insulin analog regimens in diabetes individuals with eGFR <90 mL/min was associated with significantly increased eGFR (+5.45 mL/min; P = 0.04) and a lower urinary ACR (ratio logarithm -0.67; P = 0.004) when compared to HI as a reference.Further, among the insulin analogs compared, only the regimen of lispro and Gla-100 showed a significantly increased Hb concentration (+0.68 g/dL; P = 0.03) when compared to HI as a reference standard.However, further validation of these observations is necessary to identify role of Gla-100 in delaying the progression of nephropathy and in preventing early Hb decline.

CONCLUSION
Basal bolus insulin therapy is considered the standard regimen for the management of T1DM.Although Gla-100 has a proven safety profile with similar efficacy or effectiveness to first-generation insulin analogs, including NPH and detemir, the second-generation BI analogs Gla-300 and IDeg-100 are associated with lesser GV, a longer duration of action with a flexible dosing window, and lower risk of nocturnal hypoglycemia than Gla-100.Hence, overall evidence from clinical and real-world studies suggests that when available, second-generation BIs like Gla-300 and IDeg-100 may be preferred by clinicians over first-generation BIs like Gla-100 in the treatment of T1DM.

Table 1 . Overview of Gla-100 clinical trials in T1DM. References Study Design Sample Size Trial Duration Treatment HbA1c Change from Baseline (Rec- orded in the same Order the Treat- ments have been Men- tioned) Frequency of Hypogly- cemia (Recorded in the same Order the Treat- ments have been Men- tioned) Comment
(Table1) Contd…

Frequency of Hypogly- cemia (Recorded in the same order the Treat- ments have been Men- tioned) Comment
(Table 2) Contd…